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KMID : 0043319950180050336
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Archives of Pharmacal Research
1995 Volume.18 No. 5 p.336 ~ p.339
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Exogenous D-ala Enhances the Accumulation of p-Coumaroylamino Acids in Ephedra distachya Cultures
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Song Kyung-Sik
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Abstract
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Ephedra distachya cultures have been known to accumulate two major acids (p-coumaroylgicine and -D-alanine) by D-Ala treament. When D-Ala was added together with serial concentrations of yeast-derived elicitor, the accumulation of -D-Ala was geatly increased in an additive manner. In feeding experiments, -D-Ala was incorporated into at a rate of 2.2% or 2.3% of added radioactivity, indicating that exogenous D-Ala served as a precursor of the conjugate. -L-Ala wasalso incorporated into p-CDA (0.23%) in the elicitor treated cultures. This fact suggested that at least a part of was produced from active conversion of L-Ala by the elecitation. In order to investigate a possible role of D-Ala as an elicitor of acids , cold D-Ala was added together with labeled L-Ala. Although L-Ala seemed to be incorporated into by this treatment, the incorporation ratio was too small (0.054%) to draw a clear conclusion. However, the amount of , which did not use D-Ala as a substrate, was also sightly increased by D-Ala treatment irrespective of the presence of elicitor, suggesting that exogenous D-Ala might act as an elicitor of as well as a precusor substrate of .
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KEYWORD
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Ephedra distachya, D-Ala, Elicitor, Precursor, p-Coumaroylamino acids, p-Coumaroyl-D-alanine, p-Coumaroylglycine
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